MyBioSource.com's Rabbit Anti-DPH4/DNAJC24 Antibody is a Rabbit Polyclonal antibody. This antibody has been shown to work in applications such as: Immunoprecipitation, and Western Blot.
Description
Diphthamide is a post-translationally modified histidine residue found in eukaryotic and archaeal translation elongation factor 2 (EF2). It is named after the fact that it is the target of diphtheria toxin (DT) produced by Corynebacterium diphtheriae. DT and the Pseudomonas aeruginosa exotoxin A (ETA) catalyze the ADP-ribosylation reaction on the diphthamide residue of EF2 using nicotinamide adenine dinucleotide (NAD) as the ADP-ribosyl donor. EF2 catalyzes the translocation of mRNA and peptidyl-tRNA from ribosome A site to P site. The ADP-ribosylation of EF2 inactivates it, stopping protein synthesis and leading to cell death. This modification is conserved from archaebacteria to humans. DPH4 (also known as DNAJC24, JJJ3 and ZCSL3) is 1 of several enzymes involved in synthesis of diphthamide in EEF2